Baseline oxygen consumption decreases with cortical depth: Artery09_022422_Thy1_68
[ DOI: https://doi.org/10.35077/ace-dry ]
Dataset Citation:
Fomin-Thunemann, Natalie. (2022). Baseline oxygen consumption decreases with cortical depth: Artery09_022422_Thy1_68. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/ace-dry
Abstract:
The cerebral cortex is organized in cortical layers that differ in their cellular density, composition, and wiring. Cortical laminar architecture is also readily revealed by staining for cytochrome oxidase – the last enzyme in the respiratory electron transport chain located in the inner mitochondrial membrane. It has been hypothesized that a high-density band of cytochrome oxidase in cortical layer IV reflects higher oxygen consumption under baseline (unstimulated) conditions. Here, we tested the above hypothesis using direct measurements of the partial pressure of O2 (pO2) in cortical tissue by means of 2-photon phosphorescence lifetime microscopy (2PLM). We revisited our previously developed method for extraction of the cerebral metabolic rate of O2 (CMRO2) based on 2-photon pO2 measurements around diving arterioles and applied this method to estimate baseline CMRO2 in awake mice across cortical layers. To our surprise, our results revealed a decrease in baseline CMRO2 from layer I to layer IV. This decrease of CMRO2 with cortical depth was paralleled by an increase in tissue oxygenation. Higher baseline oxygenation and cytochrome density in layer IV may serve as an O2 reserve during surges of neuronal activity or certain metabolically active brain states rather than baseline energy needs. Our study provides the first quantification of microscopically resolved CMRO2 across cortical layers as a step towards better understanding of the brain energy metabolism.
Methods:
No description found.
TechnicalInfo:
| DATASET METADATA: | |
| title | Baseline oxygen consumption decreases with cortical depth: Artery09_022422_Thy1_68 |
| Other | Phosphorescence Lifetime Oxygen Imaging |
| SPECIMEN METADATA: | |
| LocalID | Thy1_68 |
| Species | Mouse |
| NCBITaxonomy | NCBI:txid10090 |
| Age | 148.0 |
| Ageunit | Days |
| Sex | Female |
| Genotype | C57BL6 |
| OrganName | Brain |
| SampleLocalID | Artery09_022422_Thy1_68 |
| Locations | somatosensory cortex |
| INSTRUMENT METADATA: | |
| MicroscopeType | Two Photon Laser Scanning Microscopy |
| MicroscopeManufacturerAndModel | Bruker Ultima |
| ObjectiveName | Olympus UMPlanFL N, Olympus XLUMPlanFl |
| ObjectiveImmersion | Water |
| ObjectiveNA | 0.5, 0.95 |
| ObjectiveMagnification | 20x |
| DetectorType | PMT |
| IlluminationTypes | Chameleon Ultra femtosecond Ti:Sapphire laser (Coherent) |
| IMAGE METADATA: | |
| xAxis | posterior-to-anterior |
| yAxis | left-to-right |
| zAxis | superior-to-inferior |
| Number | 1, 2, red, green |
| displayColor | (1.0, 0.0, 0.0),(0.0, 1.0, 0.0) |
| Representation | 1: Oxyphor 2P; 2: FITC, |
| Flurophore | Oxyphor2P, FITC-dextran, Alexa680-dextran |
| stepSizeX | 0.58 um, 1.16 um |
| stepSizeY | 0.58 um, 1.16 um |
| stepSizeZ | 3 um |
Funding:
National Institutes of Health R01-MH111359 Microscopic Foundation of multimodal human imaging; National Institutes of Health R01-DA050159 Effects of intrinsic and drug-induced neuromodulation on functional brain imaging;
National Institutes of Health R01-NS108472 The impact of microvascular (dys)regulation on cerebral flow and oxygen heterogeneity;
National Institutes of Health U19-NS123717 Local neuronal drive and neuromodulatory control of activity in the pial neurovascular circuit
Contributors:
Fomin-Thunemann, Natalie (DataCollector) [ORCID: https://orcid.org/0000-0003-0449-4645 ] Boston UniversityMächler, Philipp (ProjectMember) [ORCID: https://orcid.org/0000-0001-7996-7684 ] University of California, San Diego
Devor, Anna (ProjectLeader) [ORCID: https://orcid.org/0000-0002-5143-3960 ] Boston University
Thunemann, Martin (ProjectMember) [ORCID: https://orcid.org/0000-0003-4139-079X ] Boston University
Related Identifiers:
IsCitedBy [ DOI : https://doi.org/10.1101/2021.10.13.464176 ];
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/e7/dd/e7dd8fe6194e1a01/Artery09_022422_Thy1_68
Dataset Download Link:
[ https: https://download.brainimagelibrary.org/e7/dd/e7dd8fe6194e1a01/Artery09_022422_Thy1_68]