Neuron Morphology data in .swc format from Patch-seq experiments in mouse cortex, Cell ID 669523148
[ DOI: https://doi.org/10.35077/act-cub ]
Dataset Citation:
Dalley, Rachel, Sorensen, Staci A., Zeng, Hongkui, Allen Institute For Brain Science. (2023). Neuron Morphology data in .swc format from Patch-seq experiments in mouse cortex, Cell ID 669523148. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/act-cub
Abstract:
Mouse cortical neurons display diverse morphology, physiology and gene expression profiles. We use a method called Patch-seq, where these three data modalities are assayed simultaneously from the same neuron, to understand how the genetic profile of mouse cortical neuron types corresponds to their morphological and electrophysiological phenotypes. This collection may contain image stacks (TIFF), reconstruction files in image space (.swc, µm), a transformed file (.swc, µm) oriented so that the y axis extends vertically towards the pia surface, and a marker file (.marker, µm) indicating nodes where dendrites were truncated (10), reconstruction was not continued (20), or surface of tissue was closest to soma (30).
Methods:
manual tracing
TechnicalInfo:
| DATASET METADATA: | |
| title | Neuron Morphology data in .swc format from Patch-seq experiments in mouse cortex, Cell ID 669523148 |
| GeneralModality | cell morphology |
| Technique | PATCH-Seq |
| SPECIMEN METADATA: | |
| LocalID | 669523148 |
| Species | mouse |
| NCBITaxonomy | NCBI:txid10090 |
| Genotype | Ndnf-IRES2-dgCre/wt;Slc32a1-IRES2-FlpO/wt;Ai65(RCFL-tdT)/wt |
| SampleLocalID | 669523148 |
| Locations | VISp2/3 |
| INSTRUMENT METADATA: | |
| MicroscopeType | NA |
| MicroscopeManufacturerAndModel | NA |
| IMAGE METADATA: | |
| xAxis | NA |
| yAxis | NA |
| zAxis | NA |
| stepSizeX | 0.114 |
| stepSizeY | 0.114 |
| stepSizeZ | 0.28 |
Funding:
No funders found.
Contributors:
Dalley, Rachel (ProjectMember) [ORCID: https://orcid.org/0000-0001-7461-7845 ] Allen Institute for Brain ScienceSorensen, Staci A. (ProjectMember) [ORCID: https://orcid.org/0000-0002-6799-2126 ] Allen Institute for Brain Science
Zeng, Hongkui (ProjectLeader) [ORCID: https://orcid.org/0000-0002-0326-5878 ] Allen Institute for Brain Science
Allen Institute For Brain Science (ResearchGroup) Allen Institute for Brain Science
Related Identifiers:
No related identifiers found.
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/03/3f/033fee1bdd563364/669523148
Dataset Download Link:
[ https: https://download.brainimagelibrary.org/03/3f/033fee1bdd563364/669523148]