Data from layers shown in Figure 4: Mouse cortex showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts

[ DOI: https://doi.org/10.35077/act-hot ]

 

Dataset Citation:

Jang, Min Jee, Gradinaru Lab. (2023). Data from layers shown in Figure 4: Mouse cortex showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/act-hot

 

Abstract:

This dataset is part of mouse cortex showing single molecule RNA transcripts of endogenous cell marker genes and systemically- delivered AAV transcripts. This allows us to quantify the tropism profiles of AAVs across genetically defined cell types.

 

Methods:

No description found.

 

TechnicalInfo:

DATASET METADATA:
title	Data from layers shown in Figure 4: Mouse cortex showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts
GeneralModality	spatial transcriptomics
Technique	other
Other	USeqFISH
SPECIMEN METADATA:
LocalID	Mouse 3
Species	Mouse
NCBITaxonomy	NCBI:txid10090
Age	unknown
Ageunit	NA
Sex	Unknown
Genotype	C57BL6
SampleLocalID	461.0
INSTRUMENT METADATA:
MicroscopeType	Confocal
MicroscopeManufacturerAndModel	Andor Dragonfly
ObjectiveImmersion	Oil
ObjectiveNA	1.3
ObjectiveMagnification	40x
DetectorType	sCMOS
DetectorModel	Zyla
SampleTemperature	RT
IMAGE METADATA:
xAxis	left-to-right
yAxis	superior-to-inferior
zAxis	posterior-to-anterior
Number	0, 1, 2, 3, 4
displayColor	no display color assigned
stepSizeX	151.0
stepSizeY	151.0
stepSizeZ	500.0

 

Funding:

National Institutes of Health 5-DP1-NS111369-04 Circuit-Specific Delivery of Large Cargo Across the Nervous Systems of Adult Mammals and Embryos via Novel Engineered Systemic Vectors;
National Institutes of Health 1-UF1-MH128336-01 Engineered AAV Identification, Validation, and Dissemination Pipeline for Brain Cell Type-Specific Manipulation Across Species;
Brain Behavior Research Foundation "28097" NARSAD Young Investigator Grant;
California Institute of Technology "NA" Biology and Biological Engineering Divisional Postdoctoral Fellowship;
Natural Sciences and Engineering Research Council of Canada "NA" "NA";
California Institute of Technology "NA" Biology and Biological Engineering Divisional Diversity Fellowship;
California Institute of Technology "NA" Schmidt Academy for Software Engineering

 

Contributors:

Jang, Min Jee (ContactPerson) [ORCID: https://orcid.org/0000-0002-1536-7177 ] California Institute of Technology
Gradinaru Lab (ResearchGroup) California Institute of Technology

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.1038/s41587-022-01648-w ];

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/ce/6e/ce6e996f8efb49e1/fig4_layer

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/ce/6e/ce6e996f8efb49e1/fig4_layer]