Data from regions shown in Figure 4: Mouse brain (cortex, striatum, thalamus, and cerebellum) showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts
[ DOI: https://doi.org/10.35077/act-how ]
Dataset Citation:
Gradinaru Lab, Jang, Min Jee. (2023). Data from regions shown in Figure 4: Mouse brain (cortex, striatum, thalamus, and cerebellum) showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/act-how
Abstract:
This dataset includes variuos regions of mouse brain (cortex, striatum, thalamus, cerebellum) showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts. This allows us to quantify the tropism profiles of AAVs across genetically defined cell types.
Methods:
No description found.
TechnicalInfo:
| DATASET METADATA: | |
| title | Data from regions shown in Figure 4: Mouse brain (cortex, striatum, thalamus, and cerebellum) showing single molecule RNA transcripts of endogenous cell marker genes and systemically-delivered AAV transcripts |
| GeneralModality | spatial transcriptomics |
| Technique | other |
| Other | USeqFISH |
| SPECIMEN METADATA: | |
| LocalID | Mouse 4 |
| Species | Mouse |
| NCBITaxonomy | NCBI:txid10090 |
| Age | unknown |
| Ageunit | NA |
| Sex | Unknown |
| Genotype | C57BL6 |
| SampleLocalID | 461.0 |
| INSTRUMENT METADATA: | |
| MicroscopeType | Confocal |
| MicroscopeManufacturerAndModel | Andor Dragonfly |
| ObjectiveImmersion | Oil |
| ObjectiveNA | 1.3 |
| ObjectiveMagnification | 40x |
| DetectorType | sCMOS |
| DetectorModel | Zyla |
| SampleTemperature | RT |
| IMAGE METADATA: | |
| xAxis | left-to-right |
| yAxis | superior-to-inferior |
| zAxis | posterior-to-anterior |
| Number | 0, 1, 2, 3, 4 |
| displayColor | no display color assigned |
| stepSizeX | 151.0 |
| stepSizeY | 151.0 |
| stepSizeZ | 500.0 |
Funding:
National Institutes of Health 5-DP1-NS111369-04 Circuit-Specific Delivery of Large Cargo Across the Nervous Systems of Adult Mammals and Embryos via Novel Engineered Systemic Vectors; National Institutes of Health 1-UF1-MH128336-01 Engineered AAV Identification, Validation, and Dissemination Pipeline for Brain Cell Type-Specific Manipulation Across Species;
Brain Behavior Research Foundation "28097" NARSAD Young Investigator Grant;
California Institute of Technology "NA" Biology and Biological Engineering Divisional Postdoctoral Fellowship;
Natural Sciences and Engineering Research Council of Canada "NA" "NA";
California Institute of Technology "NA" Biology and Biological Engineering Divisional Diversity Fellowship;
California Institute of Technology "NA" Schmidt Academy for Software Engineering
Contributors:
Jang, Min Jee (ContactPerson) [ORCID: https://orcid.org/0000-0002-1536-7177 ] California Institute of TechnologyGradinaru Lab (ResearchGroup) California Institute of Technology
Related Identifiers:
IsCitedBy [ DOI : https://doi.org/10.1038/s41587-022-01648-w ];
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/ce/6e/ce6e996f8efb49e1/fig4_region
Dataset Download Link:
[ https: https://download.brainimagelibrary.org/ce/6e/ce6e996f8efb49e1/fig4_region]