Confocal imaging of developing fly brain labeling CG14322+ PNs and 91G04+ PNs: Fly 6

[ DOI: https://doi.org/10.35077/add-met ]

 

Dataset Citation:

Wong, Kin Lam Kenneth. (2023). Confocal imaging of developing fly brain labeling CG14322+ PNs and 91G04+ PNs: Fly 6. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/add-met

 

Abstract:

This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DL1/DA3 PNs (CG14322+) and DC2 PNs (91G04+) with SNAP and Halo, respectively and examined their dendrite targeting in the developing fly brain.

 

Methods:

Fly6_brain_1 and Fly6_brain_2 refer to antennal lobe regions in the left and right hemispheres of the same brain, respectively.

 

TechnicalInfo:

DATASET METADATA:
title	Confocal imaging of developing fly brain labeling CG14322+ PNs and 91G04+ PNs: Fly 6
GeneralModality	cell morphology
Technique	other
Other	chemical labeling, immunostaining
SPECIMEN METADATA:
LocalID	Dataset_02_Fly6
Species	Fruit Fly
NCBITaxonomy	NCBI:txid7227
Age	12
Ageunit	hours after puparium formation
Sex	Unknown
Genotype	GH146-FLP/UAS-FRT10-stop-FRT10-3xHalo7-CAAX; 91G04-GAL4/CG14322-T2A-QF2 QUAS-FRT-stop-FRT-mCD8-myr-4xSNAPf
OrganName	Brain
SampleLocalID	NA
Locations	Antennal lobe
INSTRUMENT METADATA:
MicroscopeType	Confocal
MicroscopeManufacturerAndModel	ZEISS LSM 780; LSM 900 with Airyscan 2
IMAGE METADATA:
xAxis	right-to-left
yAxis	superior-to-inferior
zAxis	anterior-to-posterior
Number	1,2,red,green
displayColor	(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)
stepSizeX	0.25
stepSizeY	0.25

 

Funding:

National Institutes of Health 1-R01-DC005982-01 Assembly of the Central Olfactory Networks in Drosophila;
National Institutes of Health 1-K99-DC018830-01 Live imaging of neuron circuit assembly in Drosophila olfactory system

 

Contributors:

Wong, Kin Lam Kenneth (ProjectLeader) [ORCID: https://orcid.org/0000-0002-5597-4051 ] Stanford University
Luo, Liqun (ProjectLeader) [ORCID: https://orcid.org/0000-0001-5467-9264 ] Stanford University

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.7554/elife.85521 ];

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/47/40/4740aaaa46faaaf4/Dataset_02/Dataset_02/Fly6

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/47/40/4740aaaa46faaaf4/Dataset_02/Dataset_02/Fly6]