pBMN_spGAL4_head_posterior: Posterior head confocal imaging of fly driver line pBMN_spGAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel) - Autofluorescence from the head cuticle is in the red channel

[ DOI: https://doi.org/10.35077/add-van ]

 

Dataset Citation:

Hampel, Stefanie, Seeds, Andrew. (2023). pBMN_spGAL4_head_posterior: Posterior head confocal imaging of fly driver line pBMN_spGAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel) - Autofluorescence from the head cuticle is in the red channel. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/add-van

 

Abstract:

Confocal z-stacks (.tiff stacks) of transgenic driver lines that express in mechanosensory neurons innervating specific bristle populations on the Drosophila melanogaster head. The neurons that are targeted by each driver line were labeled by expressing mCD8::GFP under control of the UAS or LexAop promotors, and then stained using anti-GFP antibodies (green channel). Autofluorescence from the head cuticle is in the red channel. The processed images are published in Figure 3 and Figure 3 – figure supplement 1 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the driver line used in each figure panel.

 

Methods:

A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.

 

TechnicalInfo:

DATASET METADATA:
title	pBMN_spGAL4_head_posterior: Posterior head confocal imaging of fly driver line pBMN_spGAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel) - Autofluorescence from the head cuticle is in the red channel
GeneralModality	other
Technique	other
Other	GAL4 and LexA expression
SPECIMEN METADATA:
LocalID	pBMN_spGAL4_head_posterior
Species	Fruit fly
NCBITaxonomy	NCBI:txid7227
Age	5 to 8
Ageunit	Days
Sex	Male
Genotype	w; 28D07_AD (attP40); VT050279_DBD (attP2)/20XUAS-IVS- mCD8::GFP (attP2)
OrganName	Peripheral (i.e. bristle mechanosensory neurons)
SampleLocalID	20210322_VT050279_DBD_28D07_AD_20x_GFP_head_6b
Locations	Head peripheral nervous system (i.e. bristle mechanosensory neurons)
INSTRUMENT METADATA:
MicroscopeType	Confocal
MicroscopeManufacturerAndModel	Zeiss LSM 800 Axio Imager.Z2
ObjectiveName	Zeiss 20x objective (Plan-Apochromat 20x/0.8)
ObjectiveNA	0.8
ObjectiveMagnification	20x
IMAGE METADATA:
xAxis	left-to-right
yAxis	left-to-right
zAxis	left-to-right
Number	1,2,red,green
displayColor	(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)
stepSizeX	0.6239
stepSizeY	0.6239

 

Funding:

National Institutes of Health 1-RF1-NS121911-01 High-resolution synaptic and functional connectivity mapping of a neural circuit architecture underlying a behavioral sequence

 

Contributors:

Hampel, Stefanie (ProjectLeader) [ORCID: https://orcid.org/0000-0001-8287-549X ] University of Puerto Rico, Medical Sciences Campus
Seeds, Andrew (ProjectLeader) [ORCID: https://orcid.org/0000-0002-4932-6496 ] University of Puerto Rico, Medical Sciences Campus

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.1101/2023.02.11.528119 ];

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/07/91/079179b6c46b4ae7/pBMN_spGAL4_head_posterior/

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/07/91/079179b6c46b4ae7/pBMN_spGAL4_head_posterior/]