Taste_Fly_3: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown)
[ DOI: https://doi.org/10.35077/age-dry ]
Dataset Citation:
Hampel, Stefanie, Seeds, Andrew. (2023). Taste_Fly_3: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown). [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/age-dry
Methods:
A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.
Abstract:
Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were stochastically labeled using driver lines that express in specific subsets of BMNs and the multicolor flipout reagent (Nern et al., 2015). The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 6-8 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle labelled (e.g. InOc or Taste bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).
TechnicalInfo:
| DATASET METADATA: | |
| title | Taste_Fly_3: Whole brain confocal imaging of fly driver line dBMN-spGAL4 crossed with UAS-MCFO-3 responder for labeling individual neurons (single channel shown) |
| GeneralModality | other |
| Technique | other |
| Other | Stochastic labelling |
| SPECIMEN METADATA: | |
| LocalID | Taste_Fly_3 |
| Species | Fruit fly |
| NCBITaxonomy | NCBI:txid7227 |
| Age | 4 to 6 |
| Ageunit | Days |
| Sex | Male |
| Genotype | w, R57C10-FLPG5.PEST (su(Hw)attP8); VT023783_AD (attP40)/+; R11D02_DBD (attP2)/10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG (su(Hw)attP1) |
| OrganName | Brain |
| SampleLocalID | C1_20210130_11D02_DBD_VT023783_AD_MCFO_unknown_days_20x_brain_4 |
| Locations | Subesophageal zone (SEZ) |
| INSTRUMENT METADATA: | |
| MicroscopeType | Confocal |
| MicroscopeManufacturerAndModel | Zeiss LSM 800 Axio Imager.Z2 |
| ObjectiveName | Zeiss 20x objective (Plan-Apochromat 20x/0.8) |
| ObjectiveNA | 0.8 |
| ObjectiveMagnification | 20x |
| IMAGE METADATA: | |
| xAxis | left-to-right |
| yAxis | left-to-right |
| zAxis | left-to-right |
| Number | 1,red |
| displayColor | (1.0, 0.0, 0.0) |
| stepSizeX | 0.6239 |
| stepSizeY | 0.6239 |
Funding:
National Institutes of Health 1-RF1-NS121911-01 High-resolution synaptic and functional connectivity mapping of a neural circuit architecture underlying a behavioral sequence
Contributors:
Hampel, Stefanie (ProjectMember) [ORCID: https://orcid.org/0000-0001-8287-549X ] University of Puerto Rico, Medical Sciences CampusSeeds, Andrew (ProjectLeader) [ORCID: https://orcid.org/0000-0002-4932-6496 ] University of Puerto Rico, Medical Sciences Campus
Related Identifiers:
IsCitedBy [ DOI : https://doi.org/10.1101/2023.02.11.528119 ];
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/d9/ab/d9abf39817ff8d78/Taste_Fly_3
Dataset Download Link:
[ https: https://download.brainimagelibrary.org/d9/ab/d9abf39817ff8d78/Taste_Fly_3]