Ant_4_Fly_3: Whole brain confocal imaging of fly driver line C155-GAL4, UAS-nSyb.eGFP (red channel) with the indicated bristle dye filled using DiD (green channel)

[ DOI: https://doi.org/10.35077/age-gem ]

 

Dataset Citation:

Hampel, Stefanie, Alejandro, Adrián, Seeds, Andrew. (2023). Ant_4_Fly_3: Whole brain confocal imaging of fly driver line C155-GAL4, UAS-nSyb.eGFP (red channel) with the indicated bristle dye filled using DiD (green channel). [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/age-gem

 

Abstract:

Confocal z-stacks (.tiff stacks) of single labeled mechanosensory neurons that innervate specific bristles on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were labeled by dye filling from specific head bristles using the dye DiD. The processed images of the BMNs are published in Figure 4 and Figure 4 – figure supplements 1-5 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the specific bristle filled (e.g. Oc or Ant_1 bristles) and the individual fly that was tested (e.g. Fly_1 and Fly_2).

 

Methods:

A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.

 

TechnicalInfo:

DATASET METADATA:
title	Ant_4_Fly_3: Whole brain confocal imaging of fly driver line C155-GAL4, UAS-nSyb.eGFP (red channel) with the indicated bristle dye filled using DiD (green channel)
GeneralModality	other
Technique	other
Other	Bristle dye fill
SPECIMEN METADATA:
LocalID	Ant_4_Fly_3
Species	Fruit fly
NCBITaxonomy	NCBI:txid7227
Age	5 to 8
Ageunit	Days
Sex	Male
Genotype	w, C155-GAL4, UAS-nSyb.e GFP
OrganName	Brain
SampleLocalID	20190404_ANT_major_4_left_brain3_DID
Locations	Subesophageal zone (SEZ)
INSTRUMENT METADATA:
MicroscopeType	Confocal
MicroscopeManufacturerAndModel	Zeiss LSM 800 Axio Imager.Z2
ObjectiveName	Zeiss 20x objective (Plan-Apochromat 20x/0.8)
ObjectiveNA	0.8
ObjectiveMagnification	20x
IMAGE METADATA:
xAxis	left-to-right
yAxis	left-to-right
zAxis	left-to-right
Number	1,2,red,green
displayColor	(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)
stepSizeX	0.6239
stepSizeY	0.6239

 

Funding:

National Institutes of Health 1-RF1-NS121911-01 High-resolution synaptic and functional connectivity mapping of a neural circuit architecture underlying a behavioral sequence

 

Contributors:

Hampel, Stefanie (ProjectMember) [ORCID: https://orcid.org/0000-0001-8287-549X ] University of Puerto Rico, Medical Sciences Campus
Alejandro, Adrián (DataCollector) [ORCID: https://orcid.org/0009-0008-8378-2066 ] University of Puerto Rico, Medical Sciences Campus
Seeds, Andrew (ProjectLeader) [ORCID: https://orcid.org/0000-0002-4932-6496 ] University of Puerto Rico, Medical Sciences Campus

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.1101/2023.02.11.528119 ];

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/8d/97/8d97589c5e028cbf/Ant_4_Fly_3

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/8d/97/8d97589c5e028cbf/Ant_4_Fly_3]