dBMN_spGAL4_brain: Whole-brain confocal imaging of fly driver line dBMN_spGAL4 expressing mCD8::GFP, stained using GFP (green channel) and NC82 (red channel) antibodies

[ DOI: https://doi.org/10.35077/age-hut ]

 

Dataset Citation:

Hampel, Stefanie, Seeds, Andrew. (2023). dBMN_spGAL4_brain: Whole-brain confocal imaging of fly driver line dBMN_spGAL4 expressing mCD8::GFP, stained using GFP (green channel) and NC82 (red channel) antibodies. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/age-hut

 

Abstract:

Confocal z-stacks (.tiff stacks) transgenic driver lines that express in mechanosensory neurons innervating specific bristle populations on the Drosophila melanogaster head. The bristle mechanosensory neurons (BMNs) were labeled by expressing mCD8::GFP under control of the UAS or LexAop promotors, and then stained using anti-GFP (green channel) and NC82 antibodies (red channel). The processed images of the BMNs are published in Figure 3 and Figure 3 – figure supplement 1 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the driver line used in each figure panel.

 

Methods:

A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.

 

TechnicalInfo:

DATASET METADATA:
title	dBMN_spGAL4_brain: Whole-brain confocal imaging of fly driver line dBMN_spGAL4 expressing mCD8::GFP, stained using GFP (green channel) and NC82 (red channel) antibodies
GeneralModality	other
Technique	other
Other	GAL4 and LexA expression
SPECIMEN METADATA:
LocalID	dBMN_spGAL4_brain
Species	Fruit fly
NCBITaxonomy	NCBI:txid7227
Age	5 to 8
Ageunit	Days
Sex	Male
Genotype	w; VT019023_AD (attP40)/+; VT050279_DBD (attP2)/20XUAS-IVS- mCD8::GFP (attP2)
OrganName	Brain
SampleLocalID	20201217_VT050279_DBD_VT019023_AD_20x_GFP_brain_2
Locations	Subesophageal zone (SEZ)
INSTRUMENT METADATA:
MicroscopeType	Confocal
MicroscopeManufacturerAndModel	Zeiss LSM 800 Axio Imager.Z2
ObjectiveName	Zeiss 20x objective (Plan-Apochromat 20x/0.8)
ObjectiveNA	0.8
ObjectiveMagnification	20x
IMAGE METADATA:
xAxis	left-to-right
yAxis	left-to-right
zAxis	left-to-right
Number	1,2,red,green
displayColor	(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)
stepSizeX	0.6239
stepSizeY	0.6239

 

Funding:

National Institutes of Health 1-RF1-NS121911-01 High-resolution synaptic and functional connectivity mapping of a neural circuit architecture underlying a behavioral sequence

 

Contributors:

Hampel, Stefanie (ProjectLeader) [ORCID: https://orcid.org/0000-0001-8287-549X ] University of Puerto Rico, Medical Sciences Campus
Seeds, Andrew (ProjectLeader) [ORCID: https://orcid.org/0000-0002-4932-6496 ] University of Puerto Rico, Medical Sciences Campus

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.1101/2023.02.11.528119 ];

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/39/26/39265b6e029f8af0/dBMN_spGAL4_brain/

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/39/26/39265b6e029f8af0/dBMN_spGAL4_brain/]