R52A06_GAL4_head_anterior: Posterior head confocal imaging of fly driver line R52A06_GAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel). Autofluorescence from the head cuticle is in the red channel.

[ DOI: https://doi.org/10.35077/age-jet ]

 

Dataset Citation:

Seeds, Andrew, Hampel, Stefanie. (2023). R52A06_GAL4_head_anterior: Posterior head confocal imaging of fly driver line R52A06_GAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel). Autofluorescence from the head cuticle is in the red channel.. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/age-jet

 

Abstract:

Confocal z-stacks (.tiff stacks) of the R52A06-GAL4 driver line that expresses in mechanosensory neurons innervating nearly all bristle populations on the Drosophila melanogaster head. The neurons that are targeted by the driver line were labeled by expressing mCD8::GFP under control of the UAS promotor, and then stained using anti-GFP antibodies (green channel). Autofluorescence from the head cuticle is in the red channel. The processed images are published in Figure 2 and Figure 2 – figure supplement 1 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the driver line used and whether the image is of the anterior or posterior head.

 

Methods:

A full protocol describing this work is available in the associated manuscript at manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”.

 

TechnicalInfo:

DATASET METADATA:
title	R52A06_GAL4_head_anterior: Posterior head confocal imaging of fly driver line R52A06_GAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel). Autofluorescence from the head cuticle is in the red channel.
GeneralModality	other
Technique	other
Other	GAL4 expression
SPECIMEN METADATA:
LocalID	R52A06_GAL4_head_anterior
Species	Fruit fly
NCBITaxonomy	NCBI:txid7227
Age	5 to 8
Ageunit	Days
Sex	Male
Genotype	w;; R52A06_GAL4 (attP2)/20XUAS-IVS- mCD8::GFP (attP2)
OrganName	Brain
SampleLocalID	20210604_52A06_GAL4_20x_GFP_20x_head_1_stitch
Locations	Subesophageal zone (SEZ)
INSTRUMENT METADATA:
MicroscopeType	Confocal
MicroscopeManufacturerAndModel	Zeiss LSM 800 Axio Imager.Z2
ObjectiveName	Zeiss 20x objective (Plan-Apochromat 20x/0.8)
ObjectiveNA	0.8
ObjectiveMagnification	20x
IMAGE METADATA:
xAxis	left-to-right
yAxis	left-to-right
zAxis	left-to-right
Number	1,2,red,green
displayColor	(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)
stepSizeX	0.6239
stepSizeY	0.6239

 

Funding:

National Institutes of Health 1-RF1-NS121911-01 High-resolution synaptic and functional connectivity mapping of a neural circuit architecture underlying a behavioral sequence

 

Contributors:

Seeds, Andrew (ProjectLeader) [ORCID: https://orcid.org/0000-0002-4932-6496 ] University of Puerto Rico, Medical Sciences Campus
Hampel, Stefanie (ProjectLeader) [ORCID: https://orcid.org/0000-0001-8287-549X ] University of Puerto Rico, Medical Sciences Campus

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.1101/2023.02.11.528119 ];

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/a8/b0/a8b0ee011f8b0c82/R52A06_GAL4_head_anterior/

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/a8/b0/a8b0ee011f8b0c82/R52A06_GAL4_head_anterior/]