STPT imaging of aged mice with vascular or mural cell labeling

[ DOI: https://doi.org/10.35077/g.1158 ]

 

Dataset Citation:

Kim, Yongsoo, Bennett, Hannah, Chon, Uree. (2024). STPT imaging of aged mice with vascular or mural cell labeling. [ Collection / Dataset ]. Brain Image Library. https://doi.org/10.35077/g.1158

 

Abstract:

Aging is the largest risk factor for neurodegenerative disorders, and commonly associated with compromised cerebrovasculature and pericytes. However, we do not know how normal aging differentially impacts the vascular structure and function in different brain areas. Here we utilize mesoscale microscopy methods (serial two-photon tomography and light sheet microscopy) to determine detailed changes in aged cerebrovascular networks. Whole-brain vascular tracing showed an overall ~10% decrease in vascular length and branching density with ~7% increase in vascular radii in aged brains. Light sheet imaging with 3D immunolabeling revealed increased arteriole tortuosity of aged brains. Notably, vasculature and pericyte densities showed selective and significant reductions in the deep cortical layers, hippocampal network, and basal forebrain areas. This dataset is related to Bennett et al., 2024, Nature Communications, preprint: PMID: 37305850

 

Funding:

National Institutes of Health 1-RF1-MH124605-01 Establishing common coordinate framework for quantitative cell census in developing mouse brains;
National Institutes of Health 1-R01-NS108407-01 Understanding cellular architecture of the neurovascular unit and its function in the whole mouse brain

 

Contributors:

Kim, Yongsoo (ProjectLeader) [ORCID: https://orcid.org/0000-0002-4277-0279 ] Pennsylvania State University
Bennett, Hannah (ProjectManager) [ORCID: https://orcid.org/0000-0002-8939-3714 ] Pennsylvania State University
Chon, Uree (ProjectMember) [ORCID: https://orcid.org/0000-0002-5620-5430 ] Pennsylvania State University

 

Related Identifiers:

IsCitedBy [ DOI : https://doi.org/10.1101/2023.05.23.541998 ];
Requires [ URL : https://download.brainimagelibrary.org/1a/19/1a192e5a49b25526/ ];