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BIL Logo A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types

[ DOI: https://doi.org/10.35077/g.1162 ]

 

Dataset Citation:
Daigle, Tanya, Tasic, Bosiljka, Ting, Jonathan, Levi, Boaz, Ben-Simon, Yoav. (2024). A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types. [ Collection / Dataset ]. Brain Image Library. https://doi.org/10.35077/g.1162

 

Abstract:
Our goal is to develop a suite of enhancer viruses that can provide access to specific brain cell classes, subclasses or types when delivered by injection to the whole brain. The viruses will be endowed with specificity by enhancers defined experimentally from the combination of single-cell RNA-seq and single cell ATAC-seq data. The viruses will be screened for specificity by several methods. EPI datasets consist of five sagittal sections, imaged using an epifluorescent microscope. In most experiments, enhancers are used to drive expression of SYFP2 (green) with DAPI (blue) and PI (red) providing a nuclear counterstain. In experiments where the enhancer drives a recombinase, signal may appear in the red channel and the PI counterstain will be omitted. Serial Two-Photon Tomography (STPT) datasets are produced by serial sectioning of an intact brain at 100 µm along the coronal plane, followed by 2-photon imaging of the exposed surface. The resulting dataset enables qualitative evaluation of the main regions/populations labeled by each enhancer AAV across the entire brain.

 

Funding:
National Institutes of Health 1-RF1-MH121274-01 Cell class- or type-specific viruses for brain-wide labeling and neural circuit examination.;
National Institutes of Health 1-RF1-MH114126-01 Development of tools for cell-type specific labeling of human and mouse neocortical neurons ;
National Institutes of Health 5-UG3-MH120095-03 Cell type selective viral tools to interrogate and correct non-human primate and human brain circuitry;
National Institutes of Health 1-U19-MH114830-01 A comprehensive whole-brain atlas of cell types in the mouse;
Allen Institute for Brain Science AIBS Internal Award Allen Institute Internal Funding

 

Contributors:
Tasic, Bosiljka (ProjectLeader) [ORCID: https://orcid.org/0000-0002-6861-4506 ] Allen Institute for Brain Science
Ting, Jonathan (ProjectLeader) [ORCID: https://orcid.org/0000-0001-8266-0392 ] Allen Institute for Brain Science
Levi, Boaz (ProjectLeader) [ORCID: https://orcid.org/0000-0002-8346-872X ] Allen Institute for Brain Science
Ben-Simon, Yoav (ProjectLeader) [ORCID: https://orcid.org/0000-0002-7075-097X ] Allen Institute for Brain Science
Daigle, Tanya (ProjectLeader) [ORCID: https://orcid.org/0000-0001-9700-8452 ] Allen Institute for Brain Science
Dwivedi, Deepanjali (Researcher) [ORCID: https://orcid.org/0000-0001-9992-9167 ] Allen Institute for Brain Science
Way, Sharon (ProjectManager) [ORCID: https://orcid.org/0000-0002-9598-2240 ] Allen Institute for Brain Science
Thyagarajan, Bargavi (ProjectManager) [ORCID: https://orcid.org/0000-0003-3871-6410 ] Allen Institute for Brain Science
Roth, Jada (Researcher) [ORCID: https://orcid.org/0000-0001-7946-2176 ] Allen Institute for Brain Science
Huff, Sydney (Researcher) [ORCID: https://orcid.org/0000-0002-2304-6023 ] Allen Institute for Brain Science
Greisman, Noah (Researcher) [ORCID: https://orcid.org/0009-0006-2436-8151 ] Allen Institute for Brain Science
Haeseleer, Francoise (Researcher) [ORCID: https://orcid.org/0000-0003-1887-1798 ] Allen Institute for Brain Science
Opitz-Araya, Ximena (Researcher) [ORCID: https://orcid.org/0000-0003-4960-3622 ] Allen Institute for Brain Science
Omstead, Victoria (Researcher) [ORCID: https://orcid.org/0000-0002-3796-970X ] Allen Institute for Brain Science
Weed, Natalie (Researcher) [ORCID: https://orcid.org/0000-0003-0891-0327 ] Allen Institute for Brain Science
Martinez, Refugio (Researcher) [ORCID: https://orcid.org/0009-0007-3737-3862 ] Allen Institute for Brain Science
Mich, John (Researcher) [ORCID: https://orcid.org/0000-0002-1626-1139 ] Allen Institute for Brain Science
Groce, Erin (Researcher) [ORCID: https://orcid.org/0009-0008-6357-2774 ] Allen Institute for Brain Science
Bendrick, Jacqueline (Researcher) [ORCID: https://orcid.org/0000-0002-2694-9914 ] Allen Institute for Brain Science
Siverts, La’ Akea (Researcher) [ORCID: https://orcid.org/0000-0002-1385-086X ] Allen Institute for Brain Science
Walker, Miranda (Researcher) [ORCID: https://orcid.org/0000-0002-3767-1591 ] Allen Institute for Brain Science
Lenz, Garreck H. (Researcher) [ORCID: https://orcid.org/0000-0002-3233-0763 ] Allen Institute for Brain Science
Yao, Shenqin (ResearchGroup) [ORCID: https://orcid.org/0000-0003-2992-4752 ] Allen Institute for Brain Science
Donadio, Nicolas (ResearchGroup) [ORCID: https://orcid.org/0000-0002-6250-1148 ] Allen Institute for Brain Science
Newman, Dakota (ResearchGroup) [ORCID: https://orcid.org/0000-0002-3835-0853 ] Allen Institute for Brain Science
Kutsal, Rana (ResearchGroup) [ORCID: https://orcid.org/0009-0008-7190-6498 ] Allen Institute for Brain Science
Khem, Shannon (ResearchGroup) [ORCID: https://orcid.org/0009-0002-6949-1381 ] Allen Institute for Brain Science
Stewart, Kaiya (ResearchGroup) [ORCID: https://orcid.org/0000-0002-7837-2715 ] Allen Institute for Brain Science
Ronellenfitch, Kara (ResearchGroup) [ORCID: https://orcid.org/0009-0007-6221-8485 ] Allen Institute for Brain Science
Halterman, Carliana (ResearchGroup) [ORCID: https://orcid.org/0009-0006-2297-2590 ] Allen Institute for Brain Science
Larsen, Rachael (ResearchGroup) [ORCID: https://orcid.org/0000-0003-0178-003X ] Allen Institute for Brain Science
Waters, Jack (ResearchGroup) [ORCID: https://orcid.org/0000-0002-2312-4183 ] Allen Institute for Brain Science
Ransford, Shea (ResearchGroup) [ORCID: https://orcid.org/0000-0001-6093-0806 ] Allen Institute for Brain Science
Dotson, Nadezhda (ResearchGroup) [ORCID: https://orcid.org/0000-0003-3414-3176 ] Allen Institute for Brain Science
Ngo, Kiet (ResearchGroup) [ORCID: https://orcid.org/0009-0002-5548-1983 ] Allen Institute for Brain Science
Taormina, Michael J. (ResearchGroup) [ORCID: https://orcid.org/0000-0003-4634-4039 ] Allen Institute for Brain Science
Reding, Melissa (ResearchGroup) [ORCID: https://orcid.org/0000-0001-9845-1827 ] Allen Institute for Brain Science
Liang, Elizabeth (ResearchGroup) [ORCID: https://orcid.org/0009-0000-1781-6784 ] Allen Institute for Brain Science
Departee, Maxwell (ResearchGroup) [ORCID: https://orcid.org/0009-0002-3723-6474 ] Allen Institute for Brain Science
Sevigny, Joshua (ResearchGroup) [ORCID: https://orcid.org/0000-0002-9297-4800 ] Allen Institute for Brain Science
Bowlus, Jessica (ResearchGroup) [ORCID: https://orcid.org/0009-0009-7335-3855 ] Allen Institute for Brain Science
Rette, Dean (ResearchGroup) [ORCID: https://orcid.org/0009-0002-5202-6627 ] Allen Institute for Brain Science
Huang, Cindy (ResearchGroup) [ORCID: https://orcid.org/0009-0002-5386-6345 ] Allen Institute for Brain Science
Shulga, Lyudmila (ResearchGroup) [ORCID: https://orcid.org/0009-0002-9785-328X ] Allen Institute for Brain Science
Casian, Brittny (ResearchGroup) [ORCID: https://orcid.org/0009-0009-9965-0364 ] Allen Institute for Brain Science
Leibly, Madison (ResearchGroup) [ORCID: https://orcid.org/0009-0000-1277-9400 ] Allen Institute for Brain Science
Helback, Olivia (ResearchGroup) [ORCID: https://orcid.org/0009-0001-7892-6319 ] Allen Institute for Brain Science
Barcelli, Tyler (ResearchGroup) [ORCID: https://orcid.org/0009-0000-0231-1441 ] Allen Institute for Brain Science
Wood, Toren (ResearchGroup) [ORCID: https://orcid.org/0009-0009-7437-4260 ] Allen Institute for Brain Science
Boyer, Gabriella (ResearchGroup) [ORCID: https://orcid.org/0009-0007-2146-2078 ] Allen Institute for Brain Science
Smith, Kimberly (ResearchGroup) [ORCID: https://orcid.org/0000-0002-3142-1970 ] Allen Institute for Brain Science
Gary, Amanda (ResearchGroup) [ORCID: https://orcid.org/0000-0002-6397-1520 ] Allen Institute for Brain Science
Ariza, Jeanelle (ResearchGroup) [ORCID: https://orcid.org/0000-0002-0829-7887 ] Allen Institute for Brain Science
Bomben, Jasmine (ResearchGroup) [ORCID: https://orcid.org/0000-0003-3965-5373 ] Allen Institute for Brain Science
Barta, Stuard (ResearchGroup) [ORCID: https://orcid.org/0000-0001-7180-3466 ] Allen Institute for Brain Science
Ruiz, Augustin (ResearchGroup) [ORCID: https://orcid.org/0000-0002-8361-5152 ] Allen Institute for Brain Science
Pom, Christina (ResearchGroup) [ORCID: https://orcid.org/0000-0001-9057-040X ] Allen Institute for Brain Science
Malone, Jocelin (ResearchGroup) [ORCID: https://orcid.org/0000-0003-0794-4250 ] Allen Institute for Brain Science
Brouner, Krissy (ResearchGroup) [ORCID: https://orcid.org/0000-0001-5790-7154 ] Allen Institute for Brain Science
Egdorf, Tom (ResearchGroup) [ORCID: https://orcid.org/0000-0001-9059-8904 ] Allen Institute for Brain Science
Oyama, Alana (ResearchGroup) [ORCID: https://orcid.org/0000-0002-4492-0533 ] Allen Institute for Brain Science
Alaya, Angela (ResearchGroup) [ORCID: https://orcid.org/0009-0002-8512-6851 ] Allen Institute for Brain Science
Sigler, Ana (ResearchGroup) [ORCID: https://orcid.org/0000-0002-5094-8073 ] Allen Institute for Brain Science
Potekhina, Lydia (ResearchGroup) [ORCID: https://orcid.org/0000-0001-8879-7920 ] Allen Institute for Brain Science
Juneau, Zoe (ResearchGroup) [ORCID: https://orcid.org/0000-0002-8860-5260 ] Allen Institute for Brain Science

 

Related Identifiers:
IsCitedBy [ DOI : https://doi.org/10.1101/2024.06.10.597244 ];
Requires [ URL : https://download.brainimagelibrary.org/4e/fa/4efaa61008dfb900/ ];
Requires [ URL : https://download.brainimagelibrary.org/bc/59/bc59278fe0669df7/ ];