Immunofluorescence Imaging Dataset Supporting the Molecular Analysis of Network Vulnerability to α-Synuclein Pathology and PAKs as Therapeutic Targets in Parkinson’s Disease

[ DOI: https://doi.org/10.35077/g.1178 ]

 

Dataset Citation:

Kurgat, Kevin, Vatsa, Naman, Henderson, Michael X., Meyerdirk, Lindsay, Breton, Libby, Brausseur, Laura, Turner, Lisa. (2025). Immunofluorescence Imaging Dataset Supporting the Molecular Analysis of Network Vulnerability to α-Synuclein Pathology and PAKs as Therapeutic Targets in Parkinson’s Disease. [ Collection / Dataset ]. Brain Image Library. https://doi.org/10.35077/g.1178

 

Abstract:

This dataset includes all immunofluorescence images associated with the publication referenced here: DOI: [10.1101/2024.10.22.619411]. In this study, full brain α-synuclein pathology was mapped in mice over 9 months. Empirical pathology data was compared to theoretical pathology estimates from a diffusion model of pathology progression along anatomical connections. Unexplained variance in the model enabled us to derive regional vulnerability that we compared to regional gene expression. We identified gene expression patterns that relate to regional vulnerability, including 12 kinases that were enriched in vulnerable regions. Among these, an inhibitor of group II PAKs demonstrated protection from neuron death and α-synuclein pathology. The dataset also comprises immunofluorescence images from both mouse brain tissue and primary hippocampal neurons.

 

Methods:

A full protocol describing this work is available at protocols.io: dx.doi.org/10.17504/protocols.io.yxmvm3zy5l3p/v1 (injections) (perfusions) dx.doi.org/10.17504/protocols.io.bp2l6xm8klqe/v1 (tissue sectioning) dx.doi.org/10.17504/protocols.io.4r3l22y6jl1y/v2 (QUINT analysis) dx.doi.org/10.17504/protocols.io.n2bvj31jblk5/v1 (MBH post- processing) dx.doi.org/10.17504/protocols.io.5jyl82ym8l2w/v1 (immunocytochemistry)

 

Funding:

Aligning Science Across Parkinson's ASAP020616 Circuitry and Brain-Body Interactions

 

Contributors:

Breton, Libby (RelatedPerson) [ORCID: https://orcid.org/0000-0001-9968-1878 ] Van Andel Institute
Kurgat, Kevin (DataCurator) [ORCID: https://orcid.org/0000-0003-3237-7390 ] Van Andel Institute
Vatsa, Naman (DataCurator) [ORCID: https://orcid.org/0000-0003-4683-3086 ] Van Andel Institute
Henderson, Michael X. (ProjectLeader) [ORCID: https://orcid.org/0000-0001-9710-0726 ] Van Andel Institute
Meyerdirk, Lindsay (RelatedPerson) [ORCID: https://orcid.org/0000-0003-4640-9517 ] Van Andel Institute
Brausseur, Laura (RelatedPerson) [ORCID: https://orcid.org/0009-0003-5331-138X ] Van Andel Institute
Turner, Lisa (RelatedPerson) [ORCID: https://orcid.org/0000-0003-3586-8566 ] Van Andel Institute

 

Related Identifiers:

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