Whole-brain AAV Toolbox BARseq Datasets

[ DOI: https://doi.org/10.35077/g.1190 ]

 

Dataset Citation:

Tasic, Bosiljka, Ting, Jonathan, O Neill, Tulip, Ben-Simon, Yoav, Hunker, Avery, Waters, Jack, Taormina, Michael J., Hastings, Sam, Allen Institute. (2025). Whole-brain AAV Toolbox BARseq Datasets. [ Collection / Dataset ]. Brain Image Library. https://doi.org/10.35077/g.1190

 

Abstract:

Our goal is to develop enhancer virus tools that can provide access to select cell classes, subclasses, and types brain-wide. Enhancer-adeno-associated viruses (enhancer AAVs) will be evaluated for specificity and completeness of cell-type labeling using a standardized BARseq spatial transcriptomics approach. Select 20-micron sections from each AAV-injected mouse brain were subjected to in situ sequencing to detect expression of endogenous cell-type marker genes as well as exogenous virally-encoded mRNA (e.g., SYFP). For all datasets, we performed cell segmentation, registration to mouse common coordinate framework, and cell type mapping to the whole mouse brain taxonomy. This data can be used to assess the specificity and completeness of AAV vector expression within cell types and brain regions.

 

Funding:

National Institutes of Health 1-U01-MH139778-01 Enhancer AAV toolbox for cell classes and subclasses in the brains of mouse, macaque and marmoset ;
Allen Institute for Brain Science 1020106410.0 Allen Institute Internal Funding

 

Contributors:

Tasic, Bosiljka (ProjectLeader) [ORCID: https://orcid.org/0000-0002-6861-4506 ] Allen Institute for Brain Science
Ting, Jonathan (ProjectLeader) [ORCID: https://orcid.org/0000-0001-8266-0392 ] Allen Institute for Brain Science
Chen, Xiaoyin (Researcher) [ORCID: https://orcid.org/0000-0002-2807-6125 ] Allen Institute for Brain Science
Rue, Mara (Researcher) [ORCID: https://orcid.org/0000-0002-4305-7838 ] Allen Institute for Brain Science
O Neill, Tulip (Researcher) [ORCID: https://orcid.org/0009-0008-3038-0354 ] Allen Institute for Brain Science
Ben-Simon, Yoav (Researcher) [ORCID: https://orcid.org/0000-0002-7075-097X ] Allen Institute for Brain Science
Hunker, Avery (Researcher) [ORCID: https://orcid.org/0000-0003-0508-3637 ] Allen Institute for Brain Science
Waters, Jack (Researcher) [ORCID: https://orcid.org/0000-0002-2312-4183 ] Allen Institute for Brain Science
Taormina, Michael J. (Researcher) [ORCID: https://orcid.org/0000-0003-4634-4039 ] Allen Institute for Brain Science
Hastings, Sam (Researcher) [ORCID: https://orcid.org/0009-0008-9107-8597 ] Allen Institute for Brain Science
Way, Sharon (Researcher) [ORCID: https://orcid.org/0000-0002-9598-2240 ] Allen Institute for Brain Science
Thyagarajan, Bargavi (Researcher) [ORCID: https://orcid.org/0000-0003-3871-6410 ] Allen Institute for Brain Science
Hooper, Marcus (Researcher) [ORCID: https://orcid.org/0000-0003-1228-5958 ] Allen Institute for Brain Science
Dwivedi, Deepanjali (Researcher) [ORCID: https://orcid.org/0000-0001-9992-9167 ] Allen Institute for Brain Science
Isogai, Yoh (Researcher) [ORCID: https://orcid.org/0000-0001-7285-1603 ] Allen Institute for Brain Science
Khem, Shannon (Researcher) [ORCID: https://orcid.org/0009-0002-6949-1381 ] Allen Institute for Brain Science
Kutsal, Rana (Researcher) [ORCID: https://orcid.org/0009-0008-7190-6498 ] Allen Institute for Brain Science
Yao, Shenqin (Researcher) [ORCID: https://orcid.org/0000-0003-2992-4752 ] Allen Institute for Brain Science
Allen Institute (ResearchGroup) Allen Institute for Brain Science

 

Related Identifiers:

Requires [ URL : https://download.brainimagelibrary.org/1c/3d/1c3d30932cdd40b1 ];