Signature morpho-electric properties of diverse GABAergic interneurons in the human neocortex

[ DOI: https://doi.org/10.35077/g.892 ]

 

Dataset Citation:

Dalley, Rachel, Lee, Brian, Tamás, Gábor, Mansvelder, Huibert D., Ting, Jonathan, Lein, Ed, Allen Institute For Brain Science. (2023). Signature morpho-electric properties of diverse GABAergic interneurons in the human neocortex. [ Collection / Dataset ]. Brain Image Library. https://doi.org/10.35077/g.892

 

Abstract:

Human cortical interneurons have been challenging to study due to high diversity and lack of mature brain tissue platforms and genetic targeting tools. We employed rapid GABAergic neuron viral labeling plus unbiased Patch-seq sampling in brain slices to define the signature morpho-electric properties of GABAergic neurons in the human neocortex. Viral targeting greatly facilitated sampling of the SST subclass, including primate specialized double bouquet cells which mapped to two SST transcriptomic types. Multimodal analysis uncovered an SST neuron type with properties inconsistent with original subclass assignment; we instead propose reclassification into PVALB subclass. Our findings provide novel insights about functional properties of human cortical GABAergic neuron subclasses and types and highlight the essential role of multimodal annotation for refinement of emerging transcriptomic cell type taxonomies.

 

Methods:

Experimental protocols are available on protocols.io via the links listed below. Detailed descriptions of experiemntal data collection methods are also available at help.brain-map.org/display/celltypes/Documentation. Human Neurosurgical Tissue Processing: dx.doi.org/10.17504/protocols.io.bf66jrhe Patch-Seq Internal Solution with Biocytin: dx.doi.org/10.17504/protocols.io.261geo4qol47/v4 Patch-Seq Recording and Extraction: dx.doi.org/10.17504/protocols.io.bw6gphbw Post Patch Clamp Slice Fixation: dx.doi.org/10.17504/protocols.io.bg5tjy6n DAB Detection of Biocytin Labeled Tissue: dx.doi.org/10.17504/protocols.io.bg5yjy7w 20X Anatomic and Layer Identification for Biocytin Filled Cells: dx.doi.org/10.17504/protocols.io.bey9jfz6, 3D Reconstruction of Neurons in Vaa3D: dx.doi.org/10.17504/protocols.io.36wgq5683gk5/v3. Change Log: August 18 2023 - Mouse specimens were added to the dataset. See “mouse” subdirectrory for all new samples added. Added sample ID’s include: 601803754, 601810307, 602535278, 606098116, 623326230, 623415197, 623434306, 639797308, 639962984, 647045788, 652299236, 656706125, 657999088, 666141992, 668337999, 669825289, 670091720, 674069192, 682454193, 689656936, 705278421, 711770582, 713132614, 714046701, 718257664, 722821387, 740380531, 741788179, 744454952, 746200101, 752063316, 760280955, 761429710, 762590075, 762757594, 764872932, 786228558, 795563325, 809006343, 809649556, 820754704, 823253200, 841834394, 872248529, 657675492, 740890186.

 

Funding:

National Institutes of Health 1-U01-MH114812-01 A multimodal atlas of human brain cell types

 

Contributors:

Dalley, Rachel (ProjectMember) [ORCID: https://orcid.org/0000-0001-7461-7845 ] Allen Institute for Brain Science [ROR: https://ror.org/00dcv1019];
Lee, Brian (ProjectMember) [ORCID: https://orcid.org/0000-0002-3210-5638 ] Allen Institute for Brain Science [ROR: https://ror.org/00dcv1019];
Tamás, Gábor (ProjectLeader) [ORCID: https://orcid.org/0000-0002-7905-6001 ] University of Szeged [ROR: https://ror.org/01pnej532];
Mansvelder, Huibert D. (ProjectLeader) [ORCID: https://orcid.org/0000-0003-1365-5340 ] Vrije Universiteit Amsterdam [ROR: https://ror.org/008xxew50];
Ting, Jonathan (ProjectLeader) [ORCID: https://orcid.org/0000-0001-8266-0392 ] Allen Institute for Brain Science [ROR: https://ror.org/00dcv1019];
Lein, Ed (ProjectLeader) [ORCID: https://orcid.org/0000-0001-9012-6552 ] Allen Institute for Brain Science [ROR: https://ror.org/00dcv1019];
Allen Institute For Brain Science (ResearchGroup) Allen Institute for Brain Science [ROR: https://ror.org/00dcv1019]

 

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