Origin of wiring specificity in an olfactory map revealed by neuron type-specific, time-lapse imaging of dendrite targeting: Confocal imaging of developing fly brain

[ DOI: https://doi.org/10.35077/g.933 ]

 

Dataset Citation:

Wong, Kin Lam Kenneth. (2023). Origin of wiring specificity in an olfactory map revealed by neuron type-specific, time-lapse imaging of dendrite targeting: Confocal imaging of developing fly brain. [ Collection / Dataset ]. Brain Image Library. https://doi.org/10.35077/g.933

 

Abstract:

How does wiring specificity of neural maps emerge during development? Formation of the adult Drosophila olfactory glomerular map begins with patterning of projection neuron (PN) dendrites at the early pupal stage. To better understand the origin of wiring specificity of this map, we created genetic tools to systematically characterize dendrite patterning across development at PN type- specific resolution. We find that PNs use lineage and birth order combinatorially to build the initial dendritic map. Specifically, birth order directs dendrite targeting in rotating and binary manners for PNs of the anterodorsal and lateral lineages, respectively. Two-photon- and adaptive optical lattice light-sheet microscope-based time-lapse imaging reveals that PN dendrites initiate active targeting with direction-dependent branch stabilization on the timescale of seconds. Moreover, PNs that are used in both the larval and adult olfactory circuits prune their larval-specific dendrites and re-extend new dendrites simultaneously to facilitate timely olfactory map organization. Our work highlights the power and necessity of type-specific neuronal access and time- lapse imaging in identifying wiring mechanisms that underlie complex patterns of functional neural maps. This dataset is part of a fly brain imaging experiment describing dendrite targeting of projection neurons at neuronal type-specific resolution across development. Here, we labeled DL1 PNs (71B05+) and adPNs (acj6+) with Halo and GFP, DL1/DA3 PNs (CG14322+) and DC2 PNs (91G04+) with SNAP and Halo, DC3/VA1d PNs (acj6+) and DA1 PNs (acj6-) with Halo and SNAP, DL1/DA3 PNs (CG14322+) and DA1/DL3 PNs (tsh+) with SNAP and Halo, and lov+ PNs and DC2 PNs (91G04+) with GFP and Halo, respectively and examined their dendrite targeting in the developing fly brain.

 

Funding:

National Institutes of Health 1-R01-DC005982-01 Assembly of the Central Olfactory Networks in Drosophila;
National Institutes of Health 1-K99-DC018830-01 Live imaging of neuron circuit assembly in Drosophila olfactory system

 

Contributors:

Wong, Kin Lam Kenneth (ProjectLeader) [ORCID: https://orcid.org/0000-0002-5597-4051 ] Stanford University [ROR: https://ror.org/00f54p054];
Luo, Liqun (ProjectLeader) [ORCID: https://orcid.org/0000-0001-5467-9264 ] Stanford University [ROR: https://ror.org/00f54p054]

 

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