DATASET METADATA: | |
title | 597580 |
GeneralModality | connectivity |
Technique | retrograde transsynaptic tracing |
SPECIMEN METADATA: | |
LocalID | 597580 |
Species | Mouse |
NCBITaxonomy | NCBI:txid10090 |
Age | 95.0 |
Ageunit | Days |
Sex | Male |
Genotype | wt/wt |
OrganLocalID | 1135573824.0 |
OrganName | Brain |
SampleLocalID | 0539070518 |
INSTRUMENT METADATA: | |
MicroscopeType | Two Photon |
MicroscopeManufacturerAndModel | TissueVision TissueCyte 1000 |
ObjectiveName | Zeiss 20X Plan-Apochromat |
ObjectiveImmersion | Water |
ObjectiveNA | 1.0 |
ObjectiveMagnification | 20X |
DetectorType | PMT |
DetectorModel | Hamamatsu R3896 |
IlluminationWavelength | 925nm |
DetectionWavelength | 447-593nm |
SampleTemperature | 20.0 |
IMAGE METADATA: | |
xAxis | left-to-right |
yAxis | superior-to-inferior |
zAxis | posterior-to-anterior |
Number | 1,2,3,red,green,blue |
displayColor | (1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0) |
Representation | neurite |
Flurophore | CH1(background fluorescence) CH2(GFP) CH3(background fluorescence) |
stepSizeX | 0.875 |
stepSizeY | 0.875 |
stepSizeZ | 100.0 |
Channels | 3.0 |
Slices | 140.0 |
z | 1.0 |
Xsize | 16000.0 |
Ysize | 12000.0 |
Zsize | 1.0 |