BIL Logo 574638

[ DOI: https://doi.org/10.35077/sag ]

 

Dataset Citation:
Allen Institute. (2022). 574638. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/sag

 

Abstract:
This is a series of serial two-photon images of whole mouse brain to characterize cell populations for the purpose of evaluating the specificity of newly created enhancer elements

 

Methods:
No description found.

 

TechnicalInfo:
DATASET METADATA:
title 574638
GeneralModality population imaging
Technique enhancer virus labeling
SPECIMEN METADATA:
LocalID 574638
Species Mouse
NCBITaxonomy NCBI:txid10090
Age 59.0
Ageunit Days
Sex Female
Genotype wt/wt
OrganLocalID 1099072303.0
OrganName Brain
SampleLocalID 0539071361
INSTRUMENT METADATA:
MicroscopeType Two Photon
MicroscopeManufacturerAndModel TissueVision TissueCyte 1000
ObjectiveName Zeiss 20X Plan-Apochromat
ObjectiveImmersion Water
ObjectiveNA 1.0
ObjectiveMagnification 20X
DetectorType PMT
DetectorModel Hamamatsu R3896
IlluminationWavelength 925nm
DetectionWavelength 447-593nm
SampleTemperature 20.0
IMAGE METADATA:
xAxis left-to-right
yAxis superior-to-inferior
zAxis posterior-to-anterior
Number 1,2,3,red,green,blue
displayColor (1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0)
Representation neurite
Flurophore CH1(background fluorescence) CH2(GFP) CH3(background fluorescence)
stepSizeX 0.35
stepSizeY 0.35
stepSizeZ 100.0
Channels 3.0
Slices 140.0
z 1.0
Xsize 40000.0
Ysize 30000.0
Zsize 1.0

 

Funding:
National Institutes of Health 1-RF1-MH121274-01 Cell class- or type-specific viruses for brain-wide labeling and neural circuit examination

 

Contributors:
Allen Institute (ResearchGroup) Allen Institute for Brain Science
Tasic, Bosiljka (ProjectLeader) [ORCID: https://orcid.org/0000-0002-6861-4506 ] Allen Institute for Brain Science

 

Related Identifiers:
No related identifiers found.

 

Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/96/ba/96ba8210cceceeb7/0539071361

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/96/ba/96ba8210cceceeb7/0539071361]