574638
[ DOI: https://doi.org/10.35077/sag ]
Dataset Citation:
Allen Institute. (2022). 574638. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/sag
Abstract:
This is a series of serial two-photon images of whole mouse brain to characterize cell populations for the purpose of evaluating the specificity of newly created enhancer elements
Methods:
No description found.
TechnicalInfo:
| DATASET METADATA: | |
| title | 574638 |
| GeneralModality | population imaging |
| Technique | enhancer virus labeling |
| SPECIMEN METADATA: | |
| LocalID | 574638 |
| Species | Mouse |
| NCBITaxonomy | NCBI:txid10090 |
| Age | 59.0 |
| Ageunit | Days |
| Sex | Female |
| Genotype | wt/wt |
| OrganLocalID | 1099072303.0 |
| OrganName | Brain |
| SampleLocalID | 0539071361 |
| INSTRUMENT METADATA: | |
| MicroscopeType | Two Photon |
| MicroscopeManufacturerAndModel | TissueVision TissueCyte 1000 |
| ObjectiveName | Zeiss 20X Plan-Apochromat |
| ObjectiveImmersion | Water |
| ObjectiveNA | 1.0 |
| ObjectiveMagnification | 20X |
| DetectorType | PMT |
| DetectorModel | Hamamatsu R3896 |
| IlluminationWavelength | 925nm |
| DetectionWavelength | 447-593nm |
| SampleTemperature | 20.0 |
| IMAGE METADATA: | |
| xAxis | left-to-right |
| yAxis | superior-to-inferior |
| zAxis | posterior-to-anterior |
| Number | 1,2,3,red,green,blue |
| displayColor | (1.0,0.0,0.0),(0.0,1.0,0.0),(0.0,0.0,1.0) |
| Representation | neurite |
| Flurophore | CH1(background fluorescence) CH2(GFP) CH3(background fluorescence) |
| stepSizeX | 0.35 |
| stepSizeY | 0.35 |
| stepSizeZ | 100.0 |
| Channels | 3.0 |
| Slices | 140.0 |
| z | 1.0 |
| Xsize | 40000.0 |
| Ysize | 30000.0 |
| Zsize | 1.0 |
Funding:
National Institutes of Health 1-RF1-MH121274-01 Cell class- or type-specific viruses for brain-wide labeling and neural circuit examination
Contributors:
Allen Institute (ResearchGroup) Allen Institute for Brain ScienceTasic, Bosiljka (ProjectLeader) [ORCID: https://orcid.org/0000-0002-6861-4506 ] Allen Institute for Brain Science
Related Identifiers:
No related identifiers found.
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/96/ba/96ba8210cceceeb7/0539071361
Dataset Download Link:
[ https: https://download.brainimagelibrary.org/96/ba/96ba8210cceceeb7/0539071361]