CISI C57BL/6 mouse coronal sections containing MOp - October 2020 - tissue section 11
[ DOI: https://doi.org/10.35077/spa ]
Dataset Citation:
Cleary, Brian. (2022). CISI C57BL/6 mouse coronal sections containing MOp - October 2020 - tissue section 11. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/spa
Abstract:
This dataset is part of an experiment demonstrating Composite In Situ Imaging (CISI) on whole coronal sections from a bisected mouse brain. The experiment profiled 37 genes through a combination of composite measurements and validation measurements in 12 tissue sections, with the validation measurements varying in each section. Both types of measurements were made using cyclic HCR-FISH, with composite measurements consisting of multiple probes simultaneously staining with the same dye in a given round of imaging. Composite measurements were decompressed to recover predicted images for individual genes, and these were compared with validation images and used for downstream analysis (e.g. cell type clustering).
Methods:
FISH
TechnicalInfo:
| DATASET METADATA: | |
| title | CISI C57BL/6 mouse coronal sections containing MOp - October 2020 - tissue section 11 |
| GeneralModality | spatial transcriptomics |
| Technique | CISI |
| SPECIMEN METADATA: | |
| LocalID | Tissue11 |
| Species | mouse |
| NCBITaxonomy | NCBI:txid10090 |
| Age | unknown (adult) |
| OrganName | Brain |
| Locations | Coronal sections |
| INSTRUMENT METADATA: | |
| MicroscopeType | Confocal |
| MicroscopeManufacturerAndModel | Yokogawa W1 on Nikon Eclipse Ti |
| IMAGE METADATA: | |
| stepSizeX | 0.323 |
| stepSizeY | 0.323 |
| stepSizeZ | 1.5 |
Funding:
National Institutes of Health 1RF1MH121289-01 Scaling up spatial RNA profiling with compressed sensing
Contributors:
Cleary, Brian (Researcher) [ORCID: https://orcid.org/0000-0003-0825-7129 ] Broad Institute
Related Identifiers:
No related identifiers found.
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/49/77/49777378713bb584/tissue11/
Dataset Download Link:
[ https: https://download.brainimagelibrary.org/49/77/49777378713bb584/tissue11/]