MOp cell typing using BARseq

[ DOI: https://doi.org/10.35077/tan ]

 

Dataset Citation:

Chen, Xiaoyin, Sun, Yu-Chi. (2022). MOp cell typing using BARseq. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/tan

 

Abstract:

Cell-typing by in situ sequencing of cell type markers using BARseq2. This dataset is part of Sun et al., 2021 (doi.org/10.1038/s41593-021-00842-4).

 

Methods:

in situ sequencing

 

TechnicalInfo:

DATASET METADATA:
title	MOp cell typing using BARseq
GeneralModality	spatial transcriptomics
Technique	BARseq
SPECIMEN METADATA:
LocalID	XC-wt2
Species	mouse
NCBITaxonomy	NCBI:txid10090
Age	56-63
Ageunit	Days
Sex	Male
OrganName	Brain
Locations	Motor cortex
INSTRUMENT METADATA:
MicroscopeType	Spinning disk confocal
MicroscopeManufacturerAndModel	Olympus IX-81, Crest Xlight v2
IMAGE METADATA:
Number	Sequencing images: 1,2,3,4,5,G,T,A,C,DIC; Hybridization images: 1,2,3,4,5,6,GFP,YFP,TxRed,Cy5,DAPI,DIC
displayColor	(0,1,1)(1,1,0),(1,0,1)(1,1,1)(0,0,0);(0,1,1)(1,1,0)(1,0,1),(1,1,1)(0,0,1),(0,0,0)
stepSizeX	0.34/0.65
stepSizeY	0.34/0.65
stepSizeZ	1/7

 

Funding:

National Institutes of Health 5-U19-MH-114821-01 A Comprehensive Center for Mouse Brain Cell Atlas

 

Contributors:

Chen, Xiaoyin (Researcher) [ORCID: https://orcid.org/0000-0002-2807-6125 ] Allen Institute
Sun, Yu-Chi (Researcher) [ORCID: https://orcid.org/0000-0002-8014-9387 ] New York University
Zador, Anthony (ProjectLeader) [ORCID: https://orcid.org/0000-0002-8431-9136 ] Cold Spring Harbor Laboratory

 

Related Identifiers:

No related identifiers found.

 

Data location on the Brain Image Library Analysis Ecosystem:

/bil/data/06/35/0635a0b3b0954c7e/20200111M1genes/

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/06/35/0635a0b3b0954c7e/20200111M1genes/]