BIL Logo cadherin expression and projection mapping in MOp

[ DOI: https://doi.org/10.35077/tar ]

 

Dataset Citation:
Chen, Xiaoyin, Sun, Yu-Chi. (2022). cadherin expression and projection mapping in MOp. [ Dataset / Microscopy ]. Brain Image Library. https://doi.org/10.35077/tar

 

Abstract:
Correlating gene expression to projections in MOp using BARseq2. This dataset is part of Sun et al., 2021 (doi.org/10.1038/s41593-021-00842-4).

 

Methods:
in situ sequencing

 

TechnicalInfo:
DATASET METADATA:
title cadherin expression and projection mapping in MOp
GeneralModality Connectivity and spatial transcriptomics
Technique BARseq
SPECIMEN METADATA:
LocalID XC116
Species mouse
NCBITaxonomy NCBI:txid10090
Age 56-63
Ageunit Days
Sex Male
Genotype Strain_Name=C57Bl6/Agouti/Swiss
OrganName Brain
Locations Motor cortex
INSTRUMENT METADATA:
MicroscopeType Spinning disk confocal
MicroscopeManufacturerAndModel Olympus IX-81, Crest Xlight v2
IMAGE METADATA:
Number Sequencing images: 1,2,3,4,5,G,T,A,C,DIC; Hybridization images: 1,2,3,4,5,6,GFP,YFP,TxRed,Cy5,DAPI,DIC
displayColor (0,1,1)(1,1,0),(1,0,1)(1,1,1)(0,0,0);(0,1,1)(1,1,0)(1,0,1),(1,1,1)(0,0,1),(0,0,0)
stepSizeX 0.34/0.65
stepSizeY 0.34/0.65
stepSizeZ 1/7

 

Funding:
National Institutes of Health 5-U19-MH-114821-01 A Comprehensive Center for Mouse Brain Cell Atlas

 

Contributors:
Chen, Xiaoyin (Researcher) [ORCID: https://orcid.org/0000-0002-2807-6125 ] Allen Institute
Sun, Yu-Chi (Researcher) [ORCID: https://orcid.org/0000-0002-8014-9387 ] New York University
Zador, Anthony (ProjectLeader) [ORCID: https://orcid.org/0000-0002-8431-9136 ] Cold Spring Harbor Laboratory

 

Related Identifiers:
No related identifiers found.

 

Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/06/35/0635a0b3b0954c7e/20191216M1cdhs/

 

Dataset Download Link:

[ https: https://download.brainimagelibrary.org/06/35/0635a0b3b0954c7e/20191216M1cdhs/]